Surfactant-based supramolecular dye assembly: A highly selective and economically viable platform for quantification of heparin antidote.

Herein, we have employed a supramolecular assembly of a cationic dye, LDS-698 and a common surfactant sodium dodecyl sulfate (SDS) as a turn-on fluorescent sensor for protamine (Pr) detection. Addition of cationic Pr to the solution of dye-surfactant complex brings negatively charged SDS molecules together through strong electrostatic interaction, assisting aggregation of SDS way before its critical micellar concentration (CMC). These aggregates encapsulate the dye molecules within their hydrophobic region, arresting non-radiative decay channels of the excited dye. Thus, the LDS-698•SDS assembly displays substantial enhancement in fluorescence intensity that follows a nice linear trend with Pr concentration, providing limit of detection (LOD) for Pr as low as 3.84(±0.11) nM in buffer, 124.4(±6.7) nM in 1% human serum and 28.3(±0.5) nM in 100% human urine. Furthermore, high selectivity, low background signal, large stokes shift, and emission in the biologically favorable deep-red region make the studied assembly a promising platform for Pr sensing. As of our knowledge it is the first ever Pr sensory platform, using a very common surfactant (SDS), which is economically affordable and very easily available in the market. This innovative approach can replace the expensive, exotic and specialized chemicals considered for the purpose and thus showcase its potential in practical applications.

An eco-friendly one-pot spectrofluorimetric approach for the facile determination of overactive bladder drug, tolterodine: Application to dosage forms and biological fluids.

Tolterodine tartrate (TTD) was the first antimuscarinic medication developed exclusively for the treatment of overactive bladder syndrome and was approved by the FDA in 1998. As a result of the drug's extensive utilization within the local community following its authorization, there is a pressing need to develop and validate a spectrofluorometric method that is economically efficient, easily reproducible, environmentally sustainable, and possesses high sensitivity. The developed approach relies on enhancing the fluorescence intensity of TTD to reach a level 720 % higher than its initial value, achieved through the application of an aqueous sodium dodecyl sulfate (SDS) solution. A strong correlation was observed with a correlation coefficient of 0.9998 between the concentration of TTD and the fluorescence intensity within the range of 25.0-500.0 ng mL-1. This approach could be employed to quantify TTD in its pure form and to examine pharmaceutical tablets for the purposes of verifying uniform content. Additionally, it was utilized for the evaluation of TTD concentrations in spiked human plasma.

Decellularized tissue exhibits large differences of extracellular matrix properties dependent on decellularization method: novel insights from a standardized characterization on skeletal muscle.

Decellularized matrices are an attractive choice of scaffold in regenerative medicine as they can provide the necessary extracellular matrix (ECM) components, signals and mechanical properties. Various detergent-based protocols have already been proposed for decellularization of skeletal muscle tissue. However, a proper comparison is difficult due to differences in species, muscle origin and sample sizes. Moreover, a thorough evaluation of the remaining acellular matrix is often lacking. We compared an in-house developed decellularization protocol to four previously published methods in a standardized manner. Porcine skeletal muscle samples with uniform thickness were subjected to in-depth histological, ultrastructural, biochemical and biomechanical analysis. In addition, 2D and three-dimensional cytocompatibility experiments were performed. We found that the decellularization methods had a differential effect on the properties of the resulting acellular matrices. Sodium deoxycholate combined with deoxyribonuclease I was not an effective method for decellularizing thick skeletal muscle tissue. Triton X-100 in combination with trypsin, on the other hand, removed nuclear material but not cytoplasmic proteins at low concentrations. Moreover, it led to significant alterations in the biomechanical properties. Finally, sodium dodecyl sulphate (SDS) seemed most promising, resulting in a drastic decrease in DNA content without major effects on the ECM composition and biomechanical properties. Moreover, cell attachment and metabolic activity were also found to be the highest on samples decellularized with SDS. Through a newly proposed standardized analysis, we provide a comprehensive understanding of the impact of different decellularizing agents on the structure and composition of skeletal muscle. Evaluation of nuclear content as well as ECM composition, biomechanical properties and cell growth are important parameters to assess. SDS comes forward as a detergent with the best balance between all measured parameters and holds the most promise for decellularization of skeletal muscle tissue.

Interaction between Sophorolipids and ß-glucan in Aqueous Solutions.

Skin disorders, including acne vulgaris, atopic dermatitis, and rosacea, are characterized by the presence of biofilms, which are communities of microorganisms. The mechanical stability of biofilms is attributed to one of their constituents-polysaccharides-which are secreted by microorganisms. Sophorolipids are biosurfactants with biofilm disruption and removal abilities and are expected to become alternatives for classical petrochemical-based surfactants in cosmetics. In this study, we investigated the influence of sophorolipids on ß-glucan such as dispersion status, interaction mechanism, and configuration change as a model polysaccharide of biofilm in aqueous solution. Dynamic light scattering measurements showed that sophorolipids interfere with the aggregation of ß- glucan in aqueous solutions. In contrast, sodium dodecyl sulfate (SDS), which is used as a typical surfactant reference, promotes the aggregation of ß-glucan. The interaction between sophorolipids and ß-glucan were investigated using surface tension measurements and isothermal titration calorimetry (ITC). Surface tension increased only near critical micelle concentration (CMC) region of sophorolipids in the presence of ß-glucan. This suggests that the interaction occurred in the solution rather than at the air-liquid interface. Moreover, the results of ITC indicate that hydrophobic interactions were involved in this interaction. In addition, the results of optical rotation measurements indicate that sophorolipids did not unfold the triple helical structure of ß-glucan. ß-glucan dispersion was expected to be caused steric hindrance and electrostatic repulsion when sophorolipids interacted with ß-glucan via hydrophobic interactions owing to the unique molecular structure of sophorolipids attributed by a bulky sugar moiety and a carboxyl functional group. These results demonstrated unique performances of sophorolipids on ß-glucan and provided more insights on the efficacy of sophorolipids as good anti-biofilms.

Battery waste-derived magnetic Fe-Mn-Zn/C composites as an electro-Fenton-like catalyst for the degradation of sodium dodecyl sulfate surfactant.

A batch-scale electro-Fenton (EF) process was performed using graphite anode and waste battery-based Fe -Mn -Zn/C electrocatalyst coated on low-cost graphite felt cathode. The effectiveness of the EF's performance was evident with around 83.9 + 4.1% removal of 20 mg/L of sodium-dodecyl sulfate surfactant (SDS) at an optimum current density (CD) of 5.0 mA/cm2, Na2SO4 of 0.05 M, initial pH of 7.2, and electrolysis time of 180 min. Moreover, nearly 1.78-fold more removal of SDS was achieved in EF than in the electro-oxidation process operated without any catalyst. The operating cost of 0.35 $ of per m3 per order was needed to treat SDS wastewater. The remediation of SDS follows pseudo-first-order kinetics with a rate constant of 0.0095 min-1. Additionally, 90.3 + 2.1% of SDS and 57 + 2.6% of total organic carbon (TOC) removal was attained during 240 min of treatment time in secondary treated real wastewater; hence, additional 60 min of treatment time is required for effectively treating real wastewater than synthetic wastewater. Thus, EF is effective with battery waste-derived magnetic catalyst for treating wastewater containing SDS, which can lead to achieving sustainable environmental goals.

Mechanism of surfactant interactions with feline coronavirus: A physical chemistry perspective.

HYPOTHESIS: Surfactants are inexpensive chemicals with promising applications in virus inactivation, particularly for enveloped viruses. Yet, the detailed mechanisms by which surfactants deactivate coronaviruses remain underexplored. This study delves into the virucidal mechanisms of various surfactants on Feline Coronavirus (FCoV) and their potential applications against more pathogenic coronaviruses. EXPERIMENTS: By integrating virucidal activity assays with fluorescence spectroscopy, dynamic light scattering and laser Doppler electrophoresis, alongside liposome permeability experiments, we have analyzed the effects of non-ionic and ionic surfactants on viral activity. FINDINGS: The non-ionic surfactant octaethylene glycol monodecyl ether (C10EO8) inactivates the virus by disrupting the lipid envelope, whereas ionic surfactants like Sodium Dodecyl Sulfate and Cetylpyridinium Chloride predominantly affect the spike proteins, with their impact on the viral membrane being hampered by kinetic and thermodynamic constraints. FCoV served as a safe model for studying virucidal activity, offering a faster alternative to traditional virucidal assays. The study demonstrates that physicochemical techniques can expedite the screening of virucidal compounds, contributing to the design of effective disinfectant formulations. Our results not only highlight the critical role of surfactant-virus interactions but also contribute to strategic advancements in public health measures for future pandemic containment and the ongoing challenge of antimicrobial resistance.

Novel anionic surfactant-modified chlorhexidine and its potent antimicrobial properties.

Chlorhexidine dodecyl sulfate (CHX-DS) was synthesized and characterized via single-crystal X-ray diffraction (SC-XRD), 1H nuclear magnetic resonance (NMR) spectroscopy, 1H nuclear Overhauser effect spectroscopy (NOESY), and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR). The solid-state structure, comprising a 1 : 2 stoichiometric ratio of chlorhexidine cations [C22H30Cl2N10]2+ to dodecyl sulfate anions [C12H25SO4]-, is the first report of chlorhexidine isolated with a surfactant. CHX-DS exhibits broad-spectrum antibacterial activity and demonstrates superior efficacy for reducing bacteria-generated volatile sulfur compounds (VSCs) as compared to chlorhexidine gluconate (CHG). The minimum inhibitory concentrations (MICs) of CHX-DS were 7.5, 2.5, 2.5, and 10 µM for S. enterica, E. coli, S. aureus, and S. mutans, respectively. Furthermore, MIC assays for E. coli and S. mutans demonstrate that CHX-DS and CHX exhibit a statistically significant efficacy enhancement in 2.5 µM treatment as compared to CHG. CHX-DS was incorporated into SBA-15, a mesoporous silica nanoparticle (MSN) framework, and its release was qualitatively measured via UV-vis in aqueous media, which suggests its potential as an advanced functional material for drug delivery applications.

The effect of electrical stimulation on skin vulnerability to irritants.

PURPOSE: Electrical stimulation (ES) is a widely used technique in the medical field for various purposes. The effect of ES on several skin properties has been investigated; however, its effect on skin vulnerability to irritants remains unknown. This study aimed to investigate the effects of ES application on skin vulnerability to external irritants. MATERIALS AND METHODS: An experimental study on 12 healthy male subjects (Mean ± SD, 22.9 ± 3.6 years) who completed the study. The subjects were free of skin abnormalities in the volar aspect of both forearms. Three areas were allocated to each forearm and marked as areas 1, 2, and A in the treated forearm, and areas 3, 4, and B in the control forearm. ES was applied to the volar aspect of the treated forearm for 30 min three times a week, for 2 weeks. The effect of ES on skin vulnerability was investigated using 5% and 0.5% sodium lauryl sulfate (SLS) patches applied to both treated and control forearms. The skin response to irritants was evaluated using transepidermal water loss (TEWL) and a visual erythema score 24 h after patch removal. RESULTS: Compared to the control forearm, ES increased skin permeability and erythema in response to external irritants (SLS), as measured by the visual analog score (Z = 2.75, p = 0.006) and TEWL (p < 0.05), respectively. CONCLUSIONS: ES escalates skin reactions to low concentrations of irritant substances, such as SLS, in the area between the two electrodes. This emphasizes the use of this substance, and similar irritants should be avoided in areas treated with ES.

Implementing focused echocardiography and AI-supported analysis in a population-based survey in Lesotho: implications for community-based cardiovascular disease care models.

In settings where access to expert echocardiography is limited, focused echocardiography, combined with artificial intelligence (AI)-supported analysis, may improve diagnosis and monitoring of left ventricular hypertrophy (LVH). Sixteen nurses/nurse-assistants without prior experience in echocardiography underwent a 2-day hands-on intensive training to learn how to assess parasternal long axis views (PLAX) using an inexpensive hand-held ultrasound device in Lesotho, Southern Africa. Loops were stored on a cloud-drive, analyzed using deep learning algorithms at the University Hospital Basel, and afterwards confirmed by a board-certified cardiologist. The nurses/nurse-assistants obtained 756 echocardiograms. Of the 754 uploaded image files, 628 (83.3%) were evaluable by deep learning algorithms. Of those, results of 514/628 (81.9%) were confirmed by a cardiologist. Of the 126 not evaluable by the AI algorithm, 46 (36.5%) were manually evaluable. Overall, 660 (87.5%) uploaded files were evaluable and confirmed. Following short-term training of nursing cadres, a high proportion of obtained PLAX was evaluable using AI-supported analysis. This could be a basis for AI- and telemedical support in hard-to-reach areas with minimal resources.

Ultrasound assisted electrodeposition of photocatalytic antibacterial MoS2-Zn coatings controlled by sodium dodecyl sulfate.

Photocatalytic MoS2 with visible light response is considered as a promising bactericidal material owing to its non-toxicity and high antibacterial efficiency. However, photocatalysts always exist as powder, so it is difficult to settle photocatalysts on the metal surface, which limits their application in aqueous environments. To solve this problem, ultrasound and sodium dodecyl sulfate (SDS) were introduced into the co-deposition process of MoS2 and zinc matrix, so that novel MoS2-Zn coatings were obtained. In this process, ultrasound and SDS strongly promoted the dispersion and adsorption of MoS2 on the co-depositing surfaces. Then MoS2 were proved to be composited into the Zn matrix with effective structures, and the addition of SDS effectively increased the loading content of MoS2 in the MoS2-Zn coatings. Besides, the antibacterial performance of the MoS2-Zn coatings was evaluated with three typical fouling bacteria E.coli, S.aureus and B.wiedmannii. The MoS2-Zn coating showed high and broad-spectrum antibacterial properties with over 98 % inhibition rate against these three bacteria. Furthermore, it is proved that the MoS2-Zn coatings generated superoxide (·O2-) and hydroxyl radicals (·OH) under visible light, which played the dominant and subordinate roles in the antibacterial process, respectively. The MoS2-Zn coatings also showed high antibacterial stability after four "light-dark" cycles. According to the results of the attached bacteria, the MoS2-Zn coatings were considered to effectively repel the living pelagic bacteria instead of killing the attached ones, which was highly environmentally friendly. The obtained MoS2-Zn coatings were considered promising in biofilm inhibiting and marine antifouling fields.

Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from Geobacillus thermoglucosidasius SKF4.

Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius SKF4 bacterium through polymerase chain reaction (PCR). The purified recombinant SpSKF4 protease was characterized, followed by evaluation of its possible industrial applications. The analysis of the gene sequence revealed an open reading frame (ORF) consisting of 1,206 bp, coding for a protein containing 401 amino acids. The cloned gene was expressed in Escherichia coli. The molecular weight of the enzyme was measured at 28 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The partially purified enzyme has its highest activity at a pH of 10 and a temperature of 80°C. In addition, the enzyme showed a half-life of 15 h at 80°C, and there was a 60% increase in its activity at 10 mM Ca2+ concentration. The activity of the protease was completely inhibited (100%) by phenylmethylsulfonyl fluoride (PMSF); however, the addition of sodium dodecyl sulfate (SDS) resulted in a 20% increase in activity. The enzyme was also stable in various organic solvents and in certain commercial detergents. Furthermore, the enzyme exhibited strong potential for industrial use, particularly as a detergent additive and for facilitating the recovery of silver from X-ray film.

Effect of biologically active amino acids based deep eutectic solvents on sodium dodecyl sulfate: A comparative spectroscopic study.

Study the effects of three novel synthesized biologically deep eutectic solvents (DESs) on the micellar characteristics of anionic sodium dodecyl sulfate (SDS). The biologically active amino acids based three DESs synthesized have composed the 2:1 M of L-Aspartic acid (DES1), L-Tyrosine (DES2), L-Glutamine (DES3) and choline chloride, furthermore which characterized by FTIR. Surface tension, viscosity, UV-visible, fluorescence, and FTIR spectroscopy are a few of the techniques used to study the interactions of SDS within 5 and 10 wt% of three novel biological DESs in aqueous solutions. The presence and absence of 5 and 10 wt% of the three novel biological DESs in an aqueous solution is used to study the critical micelle concentration (CMC) and various interfacial characteristics including CMC, the efficiency of adsorption, the maximum surface excess concentration, the packing parameter, the minimum area per molecule, and the surface pressure at CMC, is assessed by the surface tension method. The calculated fluorescence data and those obtained using surface tension and UV-visible methods correspond well. The interactions that cause changes in the structure of the surfactant self-assemblies within aqueous DESs were investigated using FTIR technique. It is significant to highlight that the presence of unique biological DESs considerably facilitates the micellization process for SDS and the extent is more affinity for DES2 compared to DES1/DES3. The colloidal properties of DES and their combinations with water are anticipated to benefit from the current findings.

3D electro-Fenton augmented with iron-biochar particle electrodes derived from waste iron bottle caps and sugarcane bagasse for the remediation of sodium dodecyl sulphate.

The present work demonstrates a novel strategy of synthesizing iron-biochar (Fe@BCSB) composite made with the waste iron bottle cap and sugar cane bagasse for implementation in the three-dimensional electro-Fenton (3DEF) process. The catalytic ability of the Fe@BCSB composite was explored to remediate the sodium dodecyl sulphate (SDS) surfactant from wastewater at neutral pH. At the optimum operating condition of Fe@BCSB dose of 1.0 g L-1, current density of 4.66 mA cm-2, and Na2SO4 dose of 50 mM, nearly 92.7 ± 3.1% of 20 mg L-1 of SDS abatement was attained during 120 min of electrolysis time. Moreover, the Fe@BCSB showed significant recyclability up to six cycles. Besides, other organics were successfully treated with more than 85% abatement efficiency in the proposed Fe@BCSB-supported 3DEF process. The total operating cost obtained during SDS treatment was around 0.31 US$ m-3 of wastewater. The phytotoxicity test revealed the positive impact of the 3DEF-treated effluent on the germination of the Vigna radiata. The electron paramagnetic resonance conveyed •OH as the prevailing reactive species for the oxidation of SDS in the 3DEF process. Further, about 81.3 ± 3.8% of SDS and 53.7 ± 4.1% of mineralization efficacy were acquired from the real institutional sewage.

The investigation of the binding ability between sodium dodecyl sulfate and Cu (II) in urban stormwater runoff.

The simultaneous presence of heavy metals and surfactants in runoff induces complexation and ecological harm during migration. However, interactions between these pollutants are often overlooked in past studies. Thus, investigating heavy metal-surfactant complexes in runoff is imperative. In this work, Cu (II) and sodium dodecyl sulfate (SDS) were selected to investigate the interaction between heavy metals and surfactants due to the higher detected frequency in runoff. Through 1H NMR and FTIR observation of hydrogen atom nuclear displacement and functional group displacement of SDS, the change of SDS and Cu (II) complexation was obtained, and then the complexation form of Cu (II) and SDS was verified. The results showed that solution pH values and ionic strength had significant effects on the complexation of Cu (II). When the pH values increase from 3.0 to 6.0, the complexation efficiency of SDS with Cu (II) increased by 12.12% at low concentration of SDS, which may be attributed to the excessive protonation in the aqueous solution at acidic condition. The increase of ionic strength would inhibit the complexation reaction efficiency by 19.57% and finally reached the platform with concentration of NaNO3 was 0.10 mmol/L, which was mainly due to the competitive relationship between Na (I) and Cu (II). As a general filtering material in stormwater treatment measures, natural zeolite could affect the interaction between SDS and Cu (II) greatly. After the addition of SDS, the content of free Cu (II) in the zeolite-SDS-Cu (II) three-phase mixed system was significantly reduced, indicating that SDS had a positive effect on the removal of Cu (II) from runoff. This study is of great significance for investigating the migration and transformation mechanism of SDS and Cu (II) in the future and studying the control technology of storm runoff pollution.

Diameter-Dependent Competitive Adsorption of Sodium Dodecyl Sulfate and Single-Stranded DNA on Carbon Nanotubes.

The equilibrium compositions of coatings on single-wall carbon nanotubes were spectroscopically deduced for samples dispersed in dilute sodium dodecyl sulfate (SDS) and then exposed to low concentrations of ssDNA oligomers. With all studied oligomers, displacement of the SDS tended to occur at lower ssDNA concentrations for smaller diameter nanotubes than for larger diameter ones. However, the behavior varied significantly with oligomer. For example, the diameter dependence was steeper for (TAT)4 than for (ATT)4, suggesting that interstrand head-to-tail hydrogen bonding interactions play a role in SWCNT wrapping. Concentrations of ssDNA in the range of several µg/mL displace SDS from nanotubes dispersed in 1500 µg/mL SDS solutions. This effect allows the use of coating exchange to prepare ssDNA dispersions with minimal oligomer costs. Another demonstrated use exploits the structure-dependent relative coating affinities in a simple filtration method for the diameter enrichment of SWCNT mixtures.

Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS.

The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed SP2) was evaluated for sodium dodecyl sulfate or polyethylene glycol removal from Arabidopsis thaliana tryptic peptides. The robust and efficient 40-min SP2 protocol, tested for 10-ng, 250-ng, and 10-µg peptide samples, was proposed and benchmarked thoroughly against the ethyl acetate extraction protocol. The SP2 protocol on carboxylated magnetic beads proved to be the most robust approach, even for the simultaneous removal of massive sodium dodecyl sulfate (SDS) and polyethylene glycol (PEG) contaminations from AT peptide samples in respect of the LC-MS/MS data outperforming ethyl acetate extraction.

Remediation of spent engine oil-contaminated soil through biostimulation and bioaugmentation with sodium dodecyl sulphate (SDS) and indigenous hydrocarbonoclastic bacterial isolates.

Pollution caused by spent engine oil has become a major global ecological concern as it constitutes a big threat to plants, animals, microorganisms and the soil ecosystem. This study was undertaken to examine the remediation of spent engine oil-contaminated soil through biostimulation and bioaugmentation with sodium dodecyl sulphate and indigenous hydrocarbonoclastic bacterial isolates. Twelve mesocosms were organized into four groups designated G1, G2, G3 and G4 and each filled with 2.5 kg of soil samples. Each group was composed of three mesocosms to produce a triplicate setup. G1 contained pristine soil which served as a positive control. G2 contained a total petroleum hydrocarbon (TPH) of 913.333 mg/kg in the untreated oil-polluted soil which served as a negative control. G3 contained a TPH of 913.333 mg/kg in the polluted soil inoculated with indigenous hydrocarbonoclastic bacterial isolates. G4 contained a TPH of 913.333 mg/kg in the polluted soil mixed with bacterial consortium and sodium dodecyl sulphate. The level of pollution was 36.5% in the triplicate setup G2, G3 and G4. Fourier Transform Infrared spectroscopy was used to determine the degree of hydrocarbon degradation. The initial TPH value of 913.33 mg/kg was reduced by 84.44% (142 mg/kg) in soil inoculated with indigenous hydrocarbonoclastic bacterial isolates and by 88.28% (106.66 mg/kg) in biostimulated soil. Result of this study show that soil stimulation involving bacterial consortium and sodium dodecyl sulphate was more efficient than bioaugmentation strategy alone used in the remediation of spent engine oil-polluted soil.

Drug-ionic surfactant interactions: density, sound speed, spectroscopic, and electrochemical studies.

The failure of antibiotics against infectious diseases has become a global health issue due to the incessant use of antibiotics in the community and a lack of entry of new antibacterial drugs onto the market. The limited knowledge of biophysical interactions of existing antibiotics with bio-membranes is one of the major hurdles to design and develop more effective antibiotics. Surfactant systems are the simplest biological membrane models that not only mimic the cell membrane functions but are also used to investigate the biophysical interactions between pharmaceutical drugs and bio-membranes at the molecular level. In this work, volumetric and acoustic studies were used to investigate the molecular interactions of moxifloxacin (MXF), a potential antibacterial drug, with ionic surfactants (dodecyl-tri-methyl-ammonium bromide (DTAB), a cationic surfactant and sodium dodecyl sulfate (SDS), an anionic surfactant) under physiological conditions (phosphate buffer, pH 7.4) at T = 298.15-313.15 K at an interval of 5 K. Various volumetric and acoustic parameters were computed from the density and sound speed data and interpreted in terms of MXF-ionic surfactant interaction using electrostriction effect and co-sphere overlap model. Absorption spectroscopy and cyclic voltammetry were further used to determine the binding, partitioning, and related free energies of MXF with ionic micelles.

A novel theoretical strategy for predicting dissolution kinetics and mechanisms of pharmaceuticals in complex biorelevant media.

The influence mechanism of biorelevant media on the dissolution of active pharmaceutical ingredients (APIs) is the key to their formulation design. The dissolution kinetics of naproxen (NAP) and indomethacin (IND) in biorelevant media was systematically investigated. The dissolution mechanism was analyzed by chemical potential gradient model to explore the influence of surfactant type, pH and ionic strength. Hexadecyl trimethyl ammonium bromide (CTAB) is superior to sodium dodecyl sulfate (SDS) in promoting the dissolution of NAP and IND by increasing the solubility and accelerating the surface reaction processes. The electrostatic repulsion between SDS and NAP and IND with the same negative charge facilitates the diffusion of API, while the mutual attraction between CTAB and NAP and IND is not conducive to diffusion. High pH was favorable for the dissolution of acidic NAP and IND, as the simultaneous increase in solubility, surface reaction constant, and diffusion constant. High ionic strength was beneficial for the surface reaction of NAP and IND, but hindered their diffusion. It was shown that the modeling results were in conformity with the in vitro experimental data. These results are expected to provide theoretical supports for the design of biorelevant media and pharmaceutical formulations in the pharmaceutical development.

Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens.

A pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important and useful tool in point-of-care testing, particularly when aiming to decrease the unpredictable threats posed by disease outbreak. In this study, a paper device is developed to integrate the three main processes required for detecting infectious pathogens, including DNA extraction, loop-mediated isothermal amplification (LAMP), and detection. All key reagents, including sodium dodecyl sulfate (SDS), NaOH, LAMP reagents, and carmoisine, are placed on the paper device. The paper device is operated simply via sliding and folding without using any bulky equipment, and the results can be directly observed by the naked eye. The optimized concentrations of sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and carmoisine were found to be 0.1%, 0.1 M, and 0.5 mg/mL, respectively. The paper device was used to detect Enterococcus faecium at concentrations as low as 102 CFU/mL within 60 min. Also, E. faecium spiked in milk was successfully detected using the paper device, demonstrating the feasible application in real sample analysis.